SCHEDULE

We have scheduled our Programs dividing them into Day 1, Day 2, Day 3 in order to make it convenient for you to attend in absolute comfort.

25 Jan
Day 1

Keynote Forum : 1
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Biography:

Nataliya Kushnir has truly unusual and amazing path in medicine. At the age of 21 she graduated with Honors as Medical Doctor and also as a Master and Instructor in Traditional Chinese Medicine. She moved to the US to continue her studies and medical research at Stanford. She confirmed her degree in the United States, then received Allergy-Immunology Specialist title at the National Institutes of Health in Bethesda, MD and Walter Reed Army Medical Center, Washington, next 3 years, as Scientific Investigator, led stem cell and neurotransmitter research, conducted multiple studies at the National Institute of Allergy and Infectious Diseases. Dr Kushnir published 10 papers in the major clinical and scientific journals, wrote a chapter in medical textbook, presented as Invited Speaker at more than 20 national and international conferences. It was her passion for healing and wellness that brought her to the next step – she opened a private Institute of Integrative Immunology in Berkeley, CA. She is a Director of a multidisciplinary team, and Consultant seeing more than 30 new high complexity patient from all over the World monthly. Dr Kushnir is training medical residents and students at her practice. Kushnir is proud Patient’s choice award of 2008-2010 recipient. She is invited speaker at the major specialty conferences. She is dedicated community educator – she gives free lectures at the local hospitals and schools. Most recent web publication in WebMD describes Rhinitis induced by medicines.


Abstract:

We identified a group of patients with similar clinical presentation that included chronic fatigue, development of multiple drug sensitivity, skin rashes and gastrointestinal symptoms (diarrhea, constipation, abdominal distention and cramping) that correlated with sudden increase in existing allergic symptoms, or development of new environmental and food allergy. Patients were evaluated using standard skin testing and treated with the strict diet and vitamin supplementation over a period of 30 days. Subsequent skin test demonstrated conversion of the positive skin test to negative and overall improvement of the clinical scores. We proposed that we observe a new phenomenon of the hyperactive immune system (HIS), which is treatable by modification of the lifestyle and avoidance of the certain food groups that include genetically modified and chemically raised food. Observation of the patients over the average 5 year period supported our hypothesis that HIS is a new disease of the modern lifestyle, which can be treated by diet and lifestyle modifications.

Keynote Forum : 2
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Biography:

Claude Billeaud received his MD degree from the Medical University of Bordeaux ( France) in 1979 after a graduation in human cytogenetics (1976). He then studied pediatrics and has been the Clinical Assistant Director of Bordeaux University in the departments of Pediatrics, Neonatology and Intensive Care since 1983.He currently serves as a pediatrician in the neonatal unit at the Children’s Hospital of Bordeaux, as a scientific manager of Bordeaux-Marmande human milk bank, as a lecturer and head of research (HDR : Habilitation to direct research) in neonatal nutrition at the Medical University of Bordeaux.

Abstract:

Background: The main process used to pasteurize human milk is the low-temperature, long-time Holder method (HOLDER and recently investigated, the high-temperature, short-time method.Both processes lead to an appropriated inactivation of vegetative forms but are ineffective versus the bacterial spores.
Research Aims/Questions : Find a method accomplish two main objectives: inactivation of all pathogens, including spores, and preservation of the activity of milk components.

Design/Methods: Recently, a novel approach of the High Hydrostatic Pressure processes have been developed by HPBioTECH. We compared the effect of Human Milk treatment on the same samples (raw Human milk, Holder and our novel High Hydrostatic Pressure) on vegetative and spores forms of pathogens and on bioactive components (Lipase activity, Immunes proteins)
Results: a) Pathogens destructions: two main microbial strains have been selected: Staphylococcus aureus (as reference for the vegetative forms) and Bacillus cereus (as reference for spores). This research led process adapted to the a) microbial decontamination of 6 log., either for Staphylococcus aureus or Bacillus cereus, b) Human Milk bioactive components: the main components of human milk is preserved. Activity of the lipase after this treatment (close to 80%) and that of several additional components (α-lactalbumin: 96-99%: Casein: 98-100%, Lysozyme :95-100%; lactoferrin: 93-97%; sIgA: 63-64%).
Conclusions: this novel high Hydrostatic process generate microbiologically safe human milk could potentially result in important benefits for preterm infants: (i) improved assimilation of human milk, leading to daily weight and (ii) improved resistance to infections(iii) to avoid discarding 10% of contaminated by Bacillus Cereus human milk collected.

Keynote Forum : 3

Zeev Wiesman

Ben Gurion University of the Negev , Israel

Title: Magnetic resonance relaxation sensor application for quality control of food lipids oxidation

Time : 11:05 - 11:45

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Biography:

Zeev Wiesman is a full professor at the Department of Biotechnology Engineering, Faculty of Engineering Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel. He is the head of Phyto-Lipid Biotechnology Lab (PLBL). His current research is focused on the development of Facile LF 1H NMR Morphological-Chemical Relaxation Sensor Applications for cost effective Food Quality Control. Emphasis is given to application of sensorial rapid and accurate monitoring of polyunsaturated fatty acids (PUFA) oxidation in food products.

Abstract:

A major stability issue of food and other products containing lipids is their susceptibility to oxidation and the efficacy of antioxidants which are a complex function of chemical structure, multiphase morphologies, and the effect of water interfacial forces on morphology and molecular distribution. These material parameters are difficult to characterize with current analytical methods, and for this objective we recently developed 2D and 3D 1H LF NMR spin-spin (T2) and spin-matrix (T1) energy relaxation time sensorial signal analysis for chemical and morphological mapping of liquid or solids containing lipids, for mechanistic studies of lipid oxidation and antioxidant efficacy in complex food materials. We shall discuss the T1 vs. T2 graphs for lipid samples such as linseed oil, soya and rapeseed oils in comparison to their individual fatty acid components such as oleic, linoleic and linolenic oils. Furthermore we shall emphasis how the fatty acid chemical structural variations from saturated, monounsaturated or polyunsaturated alkyl chains affect the oil's chemical and internal morphological domains and resultant susceptibility to oxidation. For example we characterized the linseed oil's aggregate structure domains on a T1 vs. T2 graph and formed a dictionary of the different Time Domain peaks with different molecular sites, and compared the spectral changes of with the individual fatty acids or their due to different internal liquid crystalline type morphologies. The chemical and morphological effect on oxidation and antioxidant efficacy is shown by changes in 1H energy relaxation times on 2D and 3D graphs. An example of the 2D energy relaxation spectrum for a lipid water emulsified food product mayonnaise will be described. This smart NMR relaxation sensor opens the way to provide an efficient supportive tool to decision makers in food industry field.
Keywords: Food oils; PUFA Magnetic resonance; H relaxation; Time domain

Keynote Forum : 4
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Biography:

Monica Rosa Loizzo is Associated Professor in Food Science Technology at University of Calabria. Monica Rosa Loizzo has got specific abilities on the main separative techniques to determine the chemical composition of different food matrix. She got specific abilities on methods to evaluate the health properties of phytochemicals rich-food. Moreover, her research interest including the influence of technological processes on the overall quality of food with particular reference to compounds able to prolong the shelf-life. Her h index is 36. She is referee of several national and international Institutions and member of the Management Committee of COST Action “Eurocaroten”.

Abstract:

Citrus species are considered to be economic and renewable resource which can be used as potential source of bioactive compounds and nutritional products for functional food development. Our research group studied the chemical composition together with the evaluation of the in vitro antioxidant, hypoglicaemic and hypolipidemic potential of several Citrus species collected in Italy such as Citrus x limon var. Femminello Comune, C.  medica var. liscio Diamante, C. bergamia, C. aurantifolia C. aurantium and C. × clementina. Recently our attention moved on the recovery of bioactive compounds from Citrus waste. With the purpose to develop a functional beverage based on Citrus, C. × clementina was selected. Juice, peel and leaves extracts were investigated for their chemical composition by HPLC-DAD, antioxidant properties and inhibitory activity on enzymes linked to “diabesity”. Neohesperedin was the main abundant compounds in all investigated samples. The addition of peel and leaves extracts to the juice causes an increase in the healthy activity of the juice as seen in ?-carotene bleaching test (53,48 and 54,76% for enriched juice vs 15,06 and 17,70% for juice alone, after 30 and 60 min of incubation, respectively) as well as in ?-amylase, ?-glucosidase and lipase inhibition assays with values of 74,13, 87,56 and 58,82% for enriched juice vs 60,37, 75,12 and 43,73% for juice alone, respectively. Collectively, our results demonstrated that Citrus fruits and its by-products represent a good source of bioactive compounds that will be further explored for future development of functional products.

Speaker - 1
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Biography:

A.H.Aamir graduated from Khyber medical college Peshawar, Pakistan in 1988 and completed his postgraduate training and degree in Diabetes and Endocrinology from UK in 1995. He is Chairman of the first consensus type 2 DM guidelines of Pakistan Endocrine Society.Principal investigator for largest Diabetes prevalence Survey of Pakistan (DPS-PAK).Past president of Pakistan Endocrine Society .Vice president of South Asian Federation of Endocrine Societies .IMMEDIATE PAST President of AACE Pak chapter.He has 43 peer reviewed journal publications and his interest includes prevention of diabetes and its complications.

Abstract:

Pakistan is one of the high diabetes prevalence country in the world with current prevalence of 16.98%. Diabetes is characterized by hyper? glycemia, hyperlipidemia, and high oxidative stress. Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients. Diet and nutrition play an impor? tant role in the development and control of diabetes. Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients. In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile. Among various wheat samples, KPK15 was observed to have highest 2,2?diphenyl? 1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples. Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W. In most of the varieties, a linear correlation was observed between glycemic index and amylose contents. The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.
Keywords:antioxidant activity, diabetes, glycemic index, maize, starch, wheat.

Speaker - 2
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Biography:

Camila Ubaque is a first-year M.Sc. Food Science and Technology student in the Universidad Nacional de Colombia, Bogotá Colombia. She received a Bachelor degree in Industrial Microbiology from Universidad Pontificia Javeriana, Bogotá in 2.010. She is currently doing her thesis on  inclusion of oregano and nisin essential oil encapsulated in biocoating edible from chitosan as a conservation alternative in beef burger. Since 2016 he has been working successfully with biopackages for the food industry and is currently the leader of 13 research seedbeds

Abstract:

Ensuring food safety is a relevant issue that leads to providing innovative alternatives that naturally prolong the shelf life of food and ensure its safety. This research considers an Edible Biocoating from Chitosan which is considered natural antimicrobial, with the inclusion of oregano essential oil (AEO) and Nisin as Bacteriocin, both compounds presenting active ingredients recognized for their antimicrobial activity and their child safe products , natural to be applied in food with the aim of prolonging the shelf life in hamburger meats. To achieve this objective, the minimum inhibitory concentration (MIC) was evaluated using the factor model using different variables of Nisin and AEO in the polymer matrix of Chitosan, finding that the concentration that inhibits microbial growth using AEO was 1.5 µL and the MIC using Nisin was 3 µL against the growth of ATCC strains of Clostridium perfringens and E. coli o157: H7. These bacteria were evaluated because the preservatives that agree with this type of products are used to inhibit their growth or are related to poisonings from the consumption of hamburger meat, so the components of the biocoating are used as nitrate substitutes and preservative nitrites that had to be toxic for human consumption. Once the Biocoating was prepared, its properties were evaluated in order to determine: water vapor permeability, oxygen and carbon dioxide permeability, mechanical properties such as extensibility, breakage and particle location. The biocoating showed low permeability to water vapor and gases, greater resistance to rupture, in terms of the location of particles were the presence of AEO, encapsulated Nisina demonstrating that the polymer matrix of Chitosan serves as a vehicle of antimicrobial substance. Hamburger meats are stored in refrigeration for 8 days, analyzing physicochemical and microbiological variables according to current regulations, finding that hamburger meats with the coating have a 40% longer shelf life than hamburger meats stored without this barrier. Sensory analysis through consumer tests for preferences not present specific differences for consumers given the incidence of the components of its formulation The behavior of the loss of quality of the meat was analyzed using the model of artificial neural networks relating the variables with the appearance of negative changes in food
Keywords: Biocoating, Chitosan, oregano essential oil, Nisin, loss quality, shelf life

Speaker - 3

Raul R. Gainetdinov

Petersburg State University , Russia Federation

Title: Trace amines and trace amine-associated receptors

Time : 14:25 - 14:55

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Biography:

Raul R. Gainetdinov is the Director of the Institute of Translational Biomedicine, Saint Petersburg State University (SPBU), Russia. Prior to SPBU, Raul R. Gainetdinov was a Senior Researcher in the Department of Neuroscience and Brain Technologies at the Italian Institute of Technology in Genova, Italy (2008-2016) and an Associate Research Professor in the Department of Cell Biology, Duke University in North Carolina, USA (1996-2008), where he is currently an Adjunct Associate Professor.  In 2013-2018, he was also Professor of the Skolkovo Institute of Science and Technology (Skoltech), Moscow. Before joining the Department of Cell Biology in 1996 and becoming faculty at Duke in 2000 he conducted research at the Institute of Pharmacology Russian Academy of Medical Sciences in Moscow (1988-1996). He received a Ph.D. degree in pharmacology in 1992 from the Russian Academy of Medical Sciences and M.D. degree in 1988 from the Second Moscow Medical Institute, Moscow, Russia.  Since 2013, he is elected Chair of the subcommittee for the Dopamine receptors of the International Union of Basic and Clinical Pharmacology Committee on Receptor Nomenclature and Drug Classification (NC-IUPHAR).  As of December 2018, he has more than 220 publications in scientific journals (including Science, Nature, Cell, PNAS) and co-authored 13 patents. His papers were cited more than 21,000 times (H-index – 72, ISI Web of Science). In 2018, Raul R. Gainetdinov was included in the list of Web of Science (WOS) Highly Cited Researchers (HCR) representing top 0.1% of scientists in the world

 

Abstract:

Trace amines are endogenous compounds classically regarded as composing ?-phenylethyalmine, p-tyramine, tryptamine, p-octopamine, and some of their metabolites. They are also abundant in common foodstuffs, and can be produced and degraded by the constitutive microbiota. The ability to utilize trace amines has arisen at least twice, with unrelated receptor families present in invertebrates and vertebrates. The term trace amine was coined to reflect the low tissue levels in mammals, however, in invertebrates relatively high levels are present where they serve the role of an invertebrate adrenergic system involved in “fight or flight” responses. Vertebrates express a family of receptors termed trace amine-associated receptors (TAARs). Humans possess 6 functional receptors: TAAR1, TAAR2, TAAR5, TAAR6, TAAR8 and TAAR9. With the exception of TAAR1, all other TAAR are expressed in olfactory epithelium neurones, where they detect diverse ethological signals. Outside the olfactory system TAAR1 is the most thoroughly studied with both central and peripheral roles. TAAR1 has been already identified as a novel therapeutic target for several neuropsychiatric disorders, including schizophrenia and depression. Among other TAARs, TAAR5 represents the most interest as regard to schizophrenia and depression since it is expressed in limbic brain areas and TAAR5 knockout mice have remarkable alterations in emotional behaviors.  Further investigations of TAARs by using knockout animals and identification of natural and synthetic ligands of these receptors could bring new approaches for the treatment of neuropsychiatric disorders.
Keywords: trace amines, TAARs, receptors, neuropsychiatruc diorders, foodstuffs

Speaker - 4

Quoc Anh Bui

Wakamono Nanotechnological Research Centre , Vietnam

Title: Develop an ELISA kit to detect endosunfan and cyclodien pesticides residues in agricultural products

Time : 14:55 - 15:25

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Biography:

Bui Quoc Anh has achieved Master of Science in Agriculture at University of Sydney in 2009. After returning to Vietnam  for working, he contributed to many scientific projects regarding food satety, cosmetic and pharmacy. Food safety products such as ELISA kit, immunological test strip detecting toxin residues in food were manufactured in Vietnam. Beside, he and his research group have developed new drugs applied nanotecnology  such as nano curcumin from tumatic, nano rutin from Styphnolobium japonicum to improve human health and keep the value of Vietnamese herbal medicine.

Abstract:

The methods that popularly used to detect pesticide residues are based on High Performance Liquid Chromatography (HPLC) and gas chromatography with electron capture detector (GC-EDC). These methods seem to be inconvenient and unaffordable to the farmer or province laboratory agencies due to high cost and complicated. Recently, immunological technique such as  Enzyme linked immunoassay (ELISA) provide a simple, rapid, reliable and inexpensive mean of detection for semi-quantitative assessment of pesticide residues.A fast and simple test was developed based on ELISA technique to detect endosunfan and other pesticides belong to cyclodien group. Polyclonal antisera were raised in rabbits against immunogen developed by conjugating protein to endonsunfan hapten using an activated ester method through a spacer arm attachement. Endosunfan hapten were synthesis from reaction between 1-hydroxy chlordane and succinic anhydride. A direct competitive ELISA was designed  to detect the sensitivity and selectivity of the antibody. All the factors affecting ELISA kit were optimized: antibody dilution is 1µg/well, enzyme conjugate dilution is 0.02 µg/ml, pH 7.4. This ELISA kit show the high sensitivity with endosunfan (LOD = 0.5 ppb, IC50 = 1.0 ppb). More over, it can detect almost cyclodien pesticide group: Endosulfan sulfate (IC50 = 0.6 ppb), Aldrin (0.6 ppb), Dieldrin (0.6ppb), Chlordan (9.6 ppb), Heptachlor (0.1ppb), Endrin (5.3 ppb) and Lindane (68 ppb).

Speaker - 5

Quoc Anh Bui

Wakamono Nanotechnology Research Centre , Vietnam

Title: Development of an ELISA to detect clenbuterol in pork using a new approach for hapten design

Time : 15:25 - 15:55

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Biography:

Bui Quoc Anh has achieved Master of Science in Agriculture at University of Sydney in 2009. After returning to Vietnam  for working, he contributed to many scientific projects regarding food satety, cosmetic and pharmacy. Food safety products such as ELISA kit, immunological test strip detecting toxin residues in food were manufactured in Vietnam. Beside, he and his research group have developed new drugs applied nanotecnology  such as nano curcumin from tumatic, nano rutin from Styphnolobium japonicum to improve human health and keep the value of Vietnamese herbal medicine.


Abstract:

This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross reactivity for other β2-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. The development of this ELISA kit was sponsored by the Vietnamese Government and will be used to monitor growth promoter residues in Vietnam ’s animal products.
Keywords: ELISA; contamination; animal products

Speaker - 6
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Biography:

Puwadej Thanichanon is currently a lecturer at Department of Geography, Ramkhamhaeng University, Thailand. He was graduated PhD from University of Bern, Switzerland. His academic backgrounds were in the field of geography and sustainable development, natural resource management and environmental science. His research interests include agricultural geography, tourism geography and political geography.

Abstract:

This study investigates the effects of improved market accessibility on agricultural land use and basic wellbeing, defined by income and rice sufficiency, in Xayaburi province, Lao PDR through a meso-scale and actor-oriented approach with data collection at both district and household level. It also investigates farmers’ decision-making as it relates to regional markets. Increasing market accessibility in rural areas facilitates cash crop trade leading to agrarian change from subsistence to commercial agricultural systems. This transformation raises concerns about food security and vulnerability to market uncertainties as farmers are likely to grow cash crops intensively and in place of food crops, leading to lower food production. Meanwhile incomes from cash crop trade are highly vulnerable to market uncertainties. We found that farmers in the south of Xayaburi, where market accessibility is higher than in the north, primarily grow cash crops and do not suffer from rice insufficiency while farmers in the north, where market accessibility is lower, rely more on subsistence agriculture and have a lower level of basic wellbeing. The major factors of better basic wellbeing in the south include: (1) better market accessibility which can mitigate the risks of market uncertainty and create enough income to compensate for and overcome losses in rice production, (2) availability of more arable land due to a larger amount of level terrain which allows farmers to expand cash crop cultivation and continue growing rice at the same time, and (3) farmer strategy to keep a part of their land for growing rice to meet their minimum consumption needs and prevent the risks of rice insufficiency.
Keywords:  market accessibility, agrarian change, Land use, Wellbeing, Lao PDR

Speaker - 1
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Biography:

Tomas Komprda has master's degree in chemistry, doctorate in veterinary medicine and PhD in Animal Physiology, and is a professor of Food Technology. He is a lecturer in Human Nutrition, Functional Foods and Food Toxicology, respectively, at the Mendel University in Brno, Czech Republic. He is a member of the Lipidomic section of the Czech Society for Biochemistry and Molecular Biology of the Czech Academy of Sciences. His current research activities comprise food safety and biochemistry and molecular biology of nutrition with an emphasis on nutraceuticals, especially long-chain polyunsaturated fatty acids and their effects on decreasing the risks of chronic degenerative diseases. 


Abstract:

Statement of the problem: Wound healing properties that are ascribed to eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are based, among other things, on alleviation of inflammation. However, in vitro decreasing of the inflammatory markers is difficult to confirm in vivo. This study tested the hypothesis that dietary oils with high EPA/DHA content will optimize a cutaneous wound healing in an animal model. Methods: Laboratory rats (5 groups with 10 animals each) were fed 7 weeks either a standard control diet (C) or standard diet with 8 % of palm oil (Pa), safflower oil (S), fish oil (F) and microalga Schizochytrium oil (Sch), respectively. Twelve days after performing full-depth circular cutaneous excisions, proliferation and maturation of the healing tissue was assessed. Results: Sch-diet (high content of DHA) in comparison with the F-diet (EPA) decreased (P < 0.05) expression of the genes coding for transforming growth factor β1 and collagen 3 in the healing tissue. Sch-diet as compared to the S-diet (high content of ω-6 linoleic acid) decreased (P < 0.05) hydroxyproline concentration in the healing tissue. Amount of the collagen α-1(III) fragment, quantified by matrix-assisted-laser-desorption-ionization imaging, decreased in the sequence C > S > Sch > Pa > F (P < 0.01). Based on the semi-quantitative histological assessment, F-diet decreased collagen proliferation (Masson trichrome staining), but, similarly to Sch-diet, increased the wound extent (H&E staining) in comparison with control. Conclusions: The tested hypothesis was confirmed only partially; EPA/DHA effects on the cutaneous wound healing were equivocal, which warrants further researc

 


Speaker - 8

M.Sylvia Subapriya

Avinashilingam University ,India.

Title: Vitamin A Deficiency- Impact on maternal and infantile nutriture

Time : 17:10 - 17:40

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Biography:

M. Sylvia Subapriya M.Sc, M.Phil, B.Ed, Ph. D is Professor in the Department of Food Science and Nutrition and Former Programme Coordinator, National Service Scheme, Youth Red Cross and Red Ribbon Club, Avinashilingam University for Women, Coimbatore. She has completed Research projects in Tribal Nutrition, Oral Leukoplakia, Sports Nutrition, Vitamin A Nutrition, Gandhian foods for Health, Disease and Sports, Co Principal Investigator/Principal Investigator for the UGC Sponsored e-PG Pathshala and UNICEF SMART Survey under CMAM Initiative among < fives; Member, Editorial Board- MJSSR and JFST; Mentor, Pelatonia Student Exchange Programme, OSU, Columbus, USA; Invited speaker/chair-International Conference in several countries.

Abstract:

The magnitude of the problem of micronutrient malnutrition, particularly that of Vitamin A Deficiency (VAD) and Iron Deficiency Anemia (IDA) among pregnant women needs systematic, region wise, planned studies in order to implement region specific solutions.  Such studies will help to understand the problem better and plan programmes for the eradication of Vitamin A Deficiency in accordance with the global agreement on combating VAD. The study aims to Ten thousand pregnant women were screened for symptoms of Vitamin A Deficiency from four representative regions of Tamil Nadu. Of 600 women with clinical and subclinical deficiency, 200 each were supplemented with dietary (E1) and synthetic (E2) supplements of vitamin A and the rest were given a placebo (P) and followed up for 12months. The impact of Nutrition education was assessed on the KAP of 100 pregnant women. VAD was found to be a problem of public health significance in all the four regions. Parity was directly related to maternal body weight and inversely with serum retinol and blood haemoglobin. Micronutrient intakes increased significantly with supplementation in maternal serum and in breast milk Breast milk volume was higher in E1 while retinol levels were higher in E2. Body weight and crown heel length of infants in E1 and E2 were significantly higher than in P group. Serum retinol and blood haemoglobin levels were higher among infants in E1 and E2 while serum total proteins were higher in the infants of mothers on dietary supplement (E1). KAP of mothers increased significantly after intervention.